EC Number   |
Reference |
|---|
   3.4.22.52 | - |
643974 |
| 3.4.22.52 | a 289 bp fragment for mu-calpain is cloned into the EcoRV site of pBluescript II KS+ vector |
643972 |
| 3.4.22.52 | cloning of the 21000 Da small subunit and expression in Escherichia coli BL384 |
643991 |
| 3.4.22.52 | complete amino acid sequence of the large subunit is deduced from its cDNA sequence |
643973 |
| 3.4.22.52 | expressed in Escherichia coli BL21 cells |
708442 |
| 3.4.22.52 | expressed in Escherichia coli BL21(DE3) cells |
708490 |
| 3.4.22.52 | expression in Escherichia coli |
643989 |
| 3.4.22.52 | expression of mutant enzyme C115S in insect cell using a baculovirus system |
643978 |
| 3.4.22.52 | large catalytic subunit and two of its mutants are expressed in Escherichia coli using the baculovirus Sf9 system, the L-muCANPDELTA3 mutant lacks domain III, mutant L-muCANPDELTA4 lacks the calmodulin-like domain IV. In Sf9 cells co-expression of the inhibitor calpastatin is necessary to prevent autolysis of the L-muCANP subunit, whereas coexpression of the regulatory subunit enhances it. Only very low levels of mRNA of the truncated form L-muCANPDELTA4 are found in bacmid-transfectred Sf9 cells, and it proves impossible to isolate this mutant using the baculovirus expression system |
643976 |
| 3.4.22.52 | large subunit |
643970 |
