EC Number   |
|---|
   3.4.22.48 | co-expression of His-tagged staphopain A and its inhibitor staphostatin A in Escherichia coli strain BL21(DE3) in inclusion bodies, recombinant enzyme is toxic for Escherichia coli cells, therefore a co-expression with the enzyme inhibitor is required for recombinant enzyme production, method optimization |
| 3.4.22.48 | cysteine protease staphopain B of Staphylococcus warneri transformed into Escherichia coli strain BL21(DE3)pLysS, expression failed, mutant C21A used for recombinant protein production, expression in Escherichia coli strain BL21(DE3) |
| 3.4.22.48 | DNA and partial amino acid sequence determination and analysis |
| 3.4.22.48 | expressed in Escherichia coli |
| 3.4.22.48 | expressed in Escherichia coli strain ER2566 |
| 3.4.22.48 | expression of large amounts of active wild-type enzyme in Escherichia coli is not possible, probably due to toxicity for the host |
| 3.4.22.48 | expression of wild-type enzyme as fusion protein in Escherichia coli, hybrid construct with a N-terminal GST-moiety linked to the proenzyme via a thrombin cleavable linker |
| 3.4.22.48 | overexpressed in Staphylococcus aureus, His-tagged version expressed in Escherichia coli |
| 3.4.22.48 | overexpression of the inactive mutant in Escherichia coli BL21(DE3) as His-tagged protein |
| 3.4.22.48 | prostaphopain B, expression as GST-fusion protein in Escherichia coli strain BL21(DE3) |
