EC Number   |
Reference |
|---|
   3.4.21.B57 | expression in Escherichia coli |
727248, 728576 |
| 3.4.21.B57 | expression in Escherichia coli BL21(DE3) |
726790 |
| 3.4.21.B57 | expression in Escherichia coli of pernisine, lacking the leader sequence a fusion protein with glutathione-S-transferase |
727459 |
| 3.4.21.B57 | expression of wid-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
731774 |
| 3.4.21.B57 | overproduced in Escherichia coli in a form with a putative prosequence in inclusion bodies, solubilized in the presence of 8 M urea, and refolded and converted to an active molecule. The enzyme is refolded in a form with a putative prosequence |
726706 |
| 3.4.21.B57 | overproduction in Escherichia coli of an enzyme derivative with the mutation of the active-site serine residue to Cys (Pro-Tk-S359C), its derivatives lacking the N-propeptide (ProC-Tk-S359C) and both propeptides (Tk-S359C), and a His-tagged form of the C-propeptide (ProC*) |
727468 |
| 3.4.21.B57 | overproduction of pro-S255A in Escherichia coli BL21-Codon-Plus(DE3) |
684164 |
| 3.4.21.B57 | recombinant overexpression of and His-tagged wild-type (pernisinewt), codon-optimised (pernisineco), and codon-optimised S355A active site mutant (pernisineS355Aco), with or without additional GST-tag or maltose-binding-protein-tag, in Escherichia coli strain BL21(DE3) requires codon preference optimisation and de-novo DNA synthesis. Undetectable expression level of unmodified wild-type enzyme, method evaluation |
732679 |
| 3.4.21.B57 | the propeptide and mature domain are independently overproduced in Escherichia coli |
726708 |
| 3.4.21.B57 | Tk-S359A without N- and C-propeptides is overproduced in Escherichia coli in a soluble form |
728144 |
