EC Number   |
|---|
   3.4.21.88 | - |
| 3.4.21.88 | cDNAs encoding the LexA DNA-binding domain (residues 1-202; X) and the herpes simplex virus regulatory protein VP16 transactivation domain (residues 413-490; V and Vstop) amplified from pEG202 |
| 3.4.21.88 | expression in Escherichia coli |
| 3.4.21.88 | gene lexA, comparative genomics and analysis of the core SOS regulon |
| 3.4.21.88 | gene lexA, construction of complementing plasmid pLexA from the rcc02361 coding region and flanking sequences are amplified from SB1003 genomic DNA, digested, and cloned into plasmid pCM62 |
| 3.4.21.88 | gene lexA, overexpression of His6-tagged enzyme in Escherichia coli strain M15 |
| 3.4.21.88 | gene lexA, quantitative enzyme PCR expression analysis |
| 3.4.21.88 | gene lexA, recombinant expression of His-tagged wild-type LexA and mutant enzymes S160A, K197A, and G126D, the isolated C-terminal segment and the N-domain in Escherichia coli strain BL21(DE3) pLysS |
| 3.4.21.88 | LexA repressor is overexpressed using Escherichia coli strain JL652 |
| 3.4.21.88 | overexpression in Escherichia coli |
