EC Number   |
|---|
   3.4.21.50 | - |
| 3.4.21.50 | gene lys-C, recombinant expression gene-optimized enzyme with a short artificial N-terminal pro-peptide MGSK in Escherichia coli strains JM109(DE3) and DSM 14459 from rhamnose-inducible expression vector pJOE to 560.3 U/l activity, the pre-N-pro gene is also cloned into pJOE, resulting in expression vector pNP-2, method optimization to over 58fold increased activity, overview |
| 3.4.21.50 | gene PGN_1416, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) |
| 3.4.21.50 | overproduction and secretion into the periplasm of Escherichia coli |
| 3.4.21.50 | recombinant expression of Gly681-Ala710 deletion mutant in Porphyromonas gingivalis strain ECR368, release of enzyme Kgp catalytic domain in soluble form into the culture supernatant |
| 3.4.21.50 | the cloned gene of prepro-API is expressed in Escherichia coli, pro-API is secreted into the periplasm and activated autocatalytically |
