EC Number |
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3.4.21.38 | - |
3.4.21.38 | FXII gene sequence determination, genotyping |
3.4.21.38 | genotyping, determination and analysis of mutant K346N gene sequence, transient expression of wild-type and K346N mutant FXII in CHO-K1 cells. Quantitative RT-PCR FXII mRNA expression analysis |
3.4.21.38 | the cDNA of the zymogen domain of FXII is cloned into the pET-28a(+) vector and the plasmid is transformed into Escherichia coli strain BL21 (DE3) and overexpressed |
3.4.21.38 | the FXII promoter contains the -299/+1 region, that is sufficient for TGF-beta1 to induce FXII expression |