EC Number   |
|---|
   3.4.21.19 | - |
| 3.4.21.19 | a proform that contains a 61-amino acid N-terminal propeptide and a 218-amino acid mature domain are expressed in Escherichia coli BL21(DE3) cells |
| 3.4.21.19 | different BIGEP forms (full-length precursor, precursor without signal peptide and mature part) are expressed in Escherichia coli |
| 3.4.21.19 | expressed as soluble His-tag fusion protein in Escherichia coli |
| 3.4.21.19 | expressed in Bacillus subtilis recombinant strains with mutations in the regulatory proteins Spo0A, KinA, and Ger |
| 3.4.21.19 | expressed in enzyme deficient strain Bacillus subtilis AJ73, sporulation and enzyme expression is strongly reduced when 1% glucose is added to the culture medium at the beginning of the growth phase, no effect of glucose addition after 20 h of cultivation period, slightly enhanced activity when glucose is added after 30 h of cultivation |
| 3.4.21.19 | expressed in Escherichia coli |
| 3.4.21.19 | expressed in Escherichia coli, poor expression of native full length protein, successful expression when mature protein sequence is fused with the pro-sequence of the analogous enzyme from Staphylococcus epidermidis, successful expression of double and four point mutant in Escherichia coli |
| 3.4.21.19 | expression in Bacillus subtilis, optimization of culture medium composition for production of recombinant enzyme, recombinant enzyme is secreted to the medium from Bacillus subtilis cells, 2-5% of the enzyme remain bound to the cell wall |
