EC Number   |
|---|
   3.4.21.112 | - |
| 3.4.21.112 | cDNA encoding amino acids 17 to 997, encompassing the prodomain, catalytic domain, and cysteine-rich domain of soluble site 1 protease (sS1P) is expressed in CHO cells |
| 3.4.21.112 | expressed in Escherichia coli |
| 3.4.21.112 | expression in CHO K1 and SRD-12B cells |
| 3.4.21.112 | first 997 amino acids of human S1P lacking the C-terminal transmembrane region. Full-length sequence of human S1P. Both tagged with a C-terminal 8 x His-tag, subcloned into the entry vector pDONR201 and expressed by baculovirus expression vector system in Sf9 cells. Additionally, first 997 amino acids of human S1P lacking the C-terminal transmembrane region expressed in Sf21 cells and Trichoplusia ni egg, High Five cells |
| 3.4.21.112 | full length SKI-1 and its H249A mutant expressed in HEK-293 cells, M19 cells, HuH7 cells and Hep-G2 cells. Wild-type SKI-1 and mutants H249A, L925A and K948A expressed in SRD-12B and CHO-K1 cells. Wild-type SKI-1 or a secretable form of St-1 containing a signal peptide followed by a FLAG tag at the N terminus and a V5 tag at the C terminus overexpressed in CHO-K1 cells. rSt-1 coexpressed with protease inhibitors in CHO-K1 and Neuro-2a cells. Mutants I985A, I989L, M990L, M990A, M990I, Y994A, N995A, and double mutant Y994A/N995A expressed in CHO-K1 cells |
| 3.4.21.112 | functional expression of full-length wild-type enzyme, which undergoes complete autoprocessing, in SRD12B cells. Recombinant expression HEK-293T and CHO cells, real-time quantitative PCR expression analysis |
| 3.4.21.112 | into pcMV-S1P-Myc |
| 3.4.21.112 | overexpression of proSKI variants in HepG2 cells |
| 3.4.21.112 | pCDNA3.1-SKI/S1P expressed in CHO-K1, BHK, Vero, or SRD-12B cells |
