EC Number   |
|---|
   3.4.14.10 | - |
| 3.4.14.10 | an 8 kb NcoI fragment covering the 5'-flanking region of the TPP2 gene, including the initiation codon, is cloned into a luciferase-containing reporter vector. HEK-293 cells and NIH3T3 cells are transiently transfected with the construct. Through sequential deletions and analysis of short PCR-fragments, the promoter can be localized to a 215 bp fragment upstream of the initiation codon |
| 3.4.14.10 | expressed in Escherichia coli |
| 3.4.14.10 | expressed in Escherichia coli JM109(pTD-lac-NdeI-PTPA-L2R1) cells |
| 3.4.14.10 | expressed in Pichia pastoris |
| 3.4.14.10 | expression in Escherichia coli |
| 3.4.14.10 | expression of murine TPP2 gene optimized for high-level expression in Escherichia coli |
| 3.4.14.10 | gene tpp, DNA and amino acid sequence determination and analysis, recombinant expression of extracellular enzyme in Escherichia coli strains JM109 and XL-1 Blue |
| 3.4.14.10 | normal and alternatively spliced cDNA variant which contains an additional 39 bp, encoding 13 amino acids in the C-terminal end of the protein, expression in human kidney 293 cells |
| 3.4.14.10 | overexpression in 293-cells, enzyme forms active oligomers |
