EC Number |
---|
3.4.13.22 | - |
3.4.13.22 | expressed as a maltose-binding protein fusion protein in Escherichia coli |
3.4.13.22 | expression in Escherichia coli and clinical strain VRE |
3.4.13.22 | gene vanX, genomic organization, functional expression of the soluble enzyme in Escherichia coli BL21(DE3) |
3.4.13.22 | gene vanX, overexpression of the soluble enzyme in Escherichia coli |
3.4.13.22 | overexpression in Escherichia coli |
3.4.13.22 | overexpression is achieved by cloning aad under the control of the nisin-inducible promoter PnisA from Lactococcus lactis. The resulting plasmid (pGIM023) is introduced into Lactobacillus plantarum strain NZ7100, a WCFS1 derivative carrying the nisR and nisK regulatory genes required for nisin induction |
3.4.13.22 | overexpression of wild-type and mutants as maltose-binding-protein fusion proteins |