EC Number   |
|---|
  3.2.1.B28 | - |
| 3.2.1.B28 | expression in Escherichia coli |
| 3.2.1.B28 | expression in Escherichia coli BL21 |
| 3.2.1.B28 | expression in Escherichia coli, mutant enzymes Q150W and Q77R/Q150W |
| 3.2.1.B28 | expression in in Lactobacillus plantarum NC8 and Lactobacillus casei as hosts. Both lactobacilli harboring the pSIP409-celB vector produce active CelB in batch bioreactor cultivations while the specific CelB activity of the cell free extract is about 44% higher with Lactobacillus plantarum than with Lactobacillus casei using 4-nitrophenyl beta-galactoside as the substrate. A fedbatch bioreactor cultivation of Lactobacillus plantarum NC8 pSIP409-celB results in a specific CelB activity of 2500 nkat 4-nitrophenyl beta-D-galactopyranoside/mg protein after 28 h. A repeated dosage of the inducer (sakacin P inducing peptide) does not increase the enzyme expression further |
| 3.2.1.B28 | expression of wild-type enzyme and mutant enzymes in Escherichia coli |
| 3.2.1.B28 | expression using a baculovirus expression vector system in silkworm, Bombyx mori |
| 3.2.1.B28 | overexpressed in Escherichia coli, resulting in high-level (up to 20% of total protein) production of the enzyme |
| 3.2.1.B28 | recombinant mutant that lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449Kis expressed in Escherichia coli BL21 |
