EC Number   |
Reference |
|---|
   3.2.1.183 | - |
2280, 649940, 651736 |
| 3.2.1.183 | expressed as a His-tagged fusion protein |
720487 |
| 3.2.1.183 | expressed as a His-tagged fusion protein in Escherichia coli |
661102 |
| 3.2.1.183 | expression of recombinant wild-type and mutant enzymes |
728341 |
| 3.2.1.183 | functionally expressed in Escherichia coli, the yeast strains Saccharomyces cerevisiae and Pichia pastoris, and insect cells. In Escherichia coli, up to 2mg protein/l cell culture is expressed, in yeast cells 0.4mg/l, up to 100 mg/l, are expressed in insect cells. In all three cell systems, insoluble protein aggregates are also observed |
663299 |
| 3.2.1.183 | gene GNE |
716189 |
| 3.2.1.183 | gene gne, genotyping, transcriptional analysis of the GNE variant c.717T>G, recombinant expression of splicing mutant c.717T>G in HEK 293 cells |
754462 |
| 3.2.1.183 | gene gne, located on chromosome 9p13.3, genotyping |
748765 |
| 3.2.1.183 | gene gne, overexpresion of wild-type and mutant enzymes in HEK-293 cells, the endogenous GNE is knocked down in HEK-293 cells using GNE-specific shRNA |
748689 |
| 3.2.1.183 | gene gne, recombinant expression of N-terminally FLAG3-tagged wild-type and mutant M743T in HEK-293 cells |
748690 |
