EC Number |
---|
3.2.1.166 | - |
3.2.1.166 | expressed in Mus musculus |
3.2.1.166 | expression in CHO cells |
3.2.1.166 | expression in COS-7 cells |
3.2.1.166 | expression of an N-terminally His-tagged truncated enzyme form, residues 36-543, containing a TEV protease cleavage site, in Escherichia coli strain BL21-CodonPlus (DE3)-RIL |
3.2.1.166 | expression of the cloned cDNA in insect and mammalian cells yields 65000 Da and 50000 Da recombinant heparanase proteins. The 50000 Da enzyme represents an N-terminally processed enzyme, at least 100fold more active than the 65000 Da form |
3.2.1.166 | expression of the enzyme in Spodoptera frugiperda Sf9 cells and secretion to the cell culture medium |
3.2.1.166 | insect cell expression system is used for the generation of large amounts of recombinant protein of high specific activity. Individual subunits are cloned into baculoviral secretory vectors and coexpressed in insect cells |
3.2.1.166 | NIH3T3 and COS-7 cells stably transfected with pBK-CMV expression vectors |
3.2.1.166 | recombinant expression in insect cells |