EC Number |
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3.2.1.135 | all pul genes expressed in Escherichia coli K-12 strains, all pull genes except pulL expressed from plasmid pCHAP1217, pulL expressed from plasmid pCHAP710. Overexpression of gfp-pulL or gfp-pulM in Escherichia coli |
3.2.1.135 | Bifidobacterium breve cultured in reinforced clostridial medium, anaeorbic, 37°C. Escherichia coli cultured in Luria Bertani broth, agitated, 37°C. Lactococcus lactis cultured in M17 broth containing 0.5% glucose, 30°C for amplification of vectro pNZ8048 containing only alpha-amylase or pullulanase encoding domains, respectively. Selection of recombinant Escherichia coli with pORI19-apuB on agar containing erythromycin and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside and isopropyl-beta-D-galactopyranoside |
3.2.1.135 | development of an an hydrotetracycline-inducible expression system for expression of a neopullulanase in Bacillus subtilis |
3.2.1.135 | Escherichia coli DH5alphaF' grown in Luria-Bertani broth, 37°C with plasmid pTZ57R/T, expression in yeast Pichia pastoris with recombinant plasmid pPICZ-BK193 in Yeast Extract Peptone Dextrose |
3.2.1.135 | Escherichia coli NovaBlue and Rosetta (DE3) pLysS, cloning and expression host, respectively, with plasmid pET-20b(+), in LB broth, 37°C |
3.2.1.135 | expression in Bacillus subtilis |
3.2.1.135 | expression in bacteria |
3.2.1.135 | expression in Escherichia coli |
3.2.1.135 | expression in Escherichia coli BL21 (DE3) |
3.2.1.135 | expression in Escherichia coli CodonPlus BL21 (DE3) |