EC Number   |
|---|
   3.2.1.131 | expressed in Arabidopsis thaliana. The cell wall-targeted enzyme affects the glucuronate substitutions of xylan, which are accessible to UX1 antibody and constitute a small fraction in Arabidopsis, whereas majority of (Me)GlcA substitutions are resistant, most likely due to the shielding by acetyl groups |
| 3.2.1.131 | expressed in Escherichia coli BL21 (DE3) cells |
| 3.2.1.131 | expressed in Escherichia coli BL21(DE3) cells |
| 3.2.1.131 | expressed in Escherichia coli BL21(DE3)pLysE cells |
| 3.2.1.131 | expressed in Escherichia coli SOLR cells carrying pBS expression vectors encoding the alpha-glucuronidase gene |
| 3.2.1.131 | expressed in Pichia pastoris strain GS115 |
| 3.2.1.131 | expression as a His10-thioredoxin fusion protein in Escherichia coli |
| 3.2.1.131 | expression in Escherichia coli |
| 3.2.1.131 | expression in Escherichia coli BL21 with an N-terminal His tag |
| 3.2.1.131 | expression of the gene in Escherichia coli is very low, even in an expression plasmid construct with optimized transcription and translation start sequences. Rare codons for arginine (AGA and AGG) and isoleucine (AUA) affect the AguA from Thermothoga maritima in Escherichia coli. x03In order to raise gene expression to meet industrial requirements, the rare codons in the N-terminal region of the AguA from Thermotoga maritima are replaced, and the aguA gene is overexpressed using the heat-inducible vector pHsh. The application of the recombinant enzyme in the production of xylobioses from xylan is also described |
