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EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 3.2.1.118PH1-PH5 genes, PH2 expressed in Pichia pastoris
Display the reaction diagram Show all sequences 3.2.1.118RNA is extracted from the tegument, nucellus and cotyledon of one sweet and two bitter (D05-187 and S3067) almond genotypes throughout fruit ripening. Sequences of nine positive prunasin hydrolases are obtained from all of the genotypes by RT-PCR and cloning. The clones, from mid ripening stage, are expressed in a heterologous system in tobacco plants by agroinfiltration
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