EC Number   |
|---|
   3.1.4.52 | - |
| 3.1.4.52 | autoinducer-deficient Vibrio cholerae strain transformed with library of random Vibrio cholerae genomic fragments fused to a promoterless luciferase cassette. Verification of Quorum sensing (QS) regulation: wild-type Vibrio cholerae El and QS mutants are transformed with each candidate reporter plasmid, each candidate exhibits similar expression pattern in a luxO D47E strain (response regulator luxO) and a hapR strain (QS transcription factor HapR) that both simulate low cell density, indicating expression of each target gene is dependent on LuxO-P as well as HapR. VCA0681 is overexpressed in Vibrio cholerae luxO D47Eel: reduction of reporter expression. Deletion of either VCA0681 from the Vibrio cholerae El genome does not alter biofilm formation (transcriptional activator vps expression). Absolute level of vps expression in the (HapR minus) Vibrio cholerae El strain 100 times higher than that of wild-type (HapR minus) Vibrio cholerae C1 strain |
| 3.1.4.52 | commercial synthetic gene PA4108, DNA and amino acid sequence determination and analysis, recombinant overexpression of C- and N-terminally His-tagged enzyme in an endogenous PDE yhjH-lacking Escherichia coli strain and in strain BL21(DE3) |
| 3.1.4.52 | commercial synthetic gene PA4781, DNA and amino acid sequence determination and analysis, recombinant overexpression of wild-type and mutant C- and N-terminally His-tagged enzymes in an endogenous PDE yhjH-lacking Escherichia coli strain and in strain BL21(DE3), the REC domain of PA4781 cannot be allosterically activated by phosphorylation in the Escherichia coli background |
| 3.1.4.52 | DNA fragments encoding GGDEF (1-172 aa) and GGDEF-EAL domain (10-426 aa) amplified separately and fused to the GST coding region in pGEX-6p-1, transformation of Escherichia coli BL21. Deletion of Xcc1959 in Xcc strain XC1 or 8004 had no significant effect on virulence factor production in vitro |
| 3.1.4.52 | ectopic expression of 31 of the conserved genes from 20 genomes of Clostridium difficile for comparison of their effect on motility and biofilm formation. Most of the PDEs are active in a Vibrio cholerae model |
| 3.1.4.52 | expressed in Escherichia coli BL21pLysE (DE3) cells |
| 3.1.4.52 | expressed in Escherichia coli strain BL21 |
| 3.1.4.52 | expression in Escherichia coli with His-tag |
| 3.1.4.52 | expression in Escherichia coli, His-tag |
