EC Number   |
Reference |
|---|
   3.1.3.67 | - |
705669 |
| 3.1.3.67 | adenovirus expressing PTEN wild-type, encoding full-length human wild-type PTEN cDNA and AdPTENC/S, encoding a dominant negative human PTEN cDNA mutant (cysteine 124 changed to serine within the catalytic domain) are used for the transduction of cell cultures, overexpression of PTEN and its mutant |
692556 |
| 3.1.3.67 | By crossing CD18-Cre recombinase transgenic mice with mice with a conditional point mutation of the pten gene, pten gene is specifically deleted in the B-cell lineage. Mutant animals do not develop B-cell malignancies. |
694494 |
| 3.1.3.67 | C-terminal domain of enzyme is expressed in BL21 (DE3) Escherichia coli cells as glutathione S-transferase-protein. Par-3/PDZ3-PTEN peptide single chain fusion protein contains a thrombin cleavage site (LVPRGS) between the C-terminus of PDZ3 domain and the PTEN peptide (DEDQSHQITKV), production of a canine knockdown |
693117 |
| 3.1.3.67 | cDNA is subcloned into bicistronic pIRES vector, which also codes for GFP expression and transiently transfected into the following cell lines: HaCaT, MCA3D, NIH 3T3, 3T3 Ki ras and 3T3 v-src |
653397 |
| 3.1.3.67 | Conditionally deleted pten in oocytes using transgenic mice expressing Cre recombinase under the control of the growth differentiation factor 9 promoter. Pten deficiency in murine oocytes causes the entire oocyte pool to become activated in life. |
694494 |
| 3.1.3.67 | Creating of a conditional mutant with a combined deletion of Smad4 and Pten, the double mutant shows skin tumor onset |
694494 |
| 3.1.3.67 | crossing of mice expressing Cre recombinase under the control of the nestin promoter to conditional PTEN mice, mice show a continous increase in brain size throughout embryonal development, individual cells from mutant brain are larger than that of wild type brains |
694494 |
| 3.1.3.67 | cytosolic domain (amino acids 215522) is used for analysis |
732809 |
| 3.1.3.67 | cytosolic domain (amino acids 248-576) is used for analysis |
732809 |
