EC Number   |
|---|
   3.1.3.62 | cDNA encoding residues 19-445 of avian MINPP or residues 31-483 of human MINPP lacking the N-terminal ER targeting sequences and the C-terminal ER retention signals are amplified by PCR and cloned into pGEM Tvector and transformed into Escherichia coli |
| 3.1.3.62 | cloned in Escherichia coli |
| 3.1.3.62 | expressed in Escherichia coli |
| 3.1.3.62 | expressed in Pichia pastoris |
| 3.1.3.62 | full-length Minpp cDNA is cloned and stably overexpressed in murine ATDC-5 cells at 2fold higher levels than in wild-type ATDC-5 cells, cellular effects of overexpression |
| 3.1.3.62 | His-tag fusion, expression in Pichia pastoris |
| 3.1.3.62 | located on chromosome 10q23, genomic organization |
| 3.1.3.62 | MINPP gene encoding multiple inositol polyphosphate phosphatase |
| 3.1.3.62 | MIPP cDNA from Jurkat T-cells is cloned and expressed in Escherichia coli M15 without the ER-targeting domain, full-length sequence |
| 3.1.3.62 | MIPP cDNA from liver and kidney is cloned and expressed in Escherichia coli M15, full-length sequence |
