EC Number |
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3.1.3.36 | 72-5ptase is expressed as a hemagglutinin (HA) tagged fusion protein in 3T3-L1 cells. Overexpression in adipocytes generates phosphatidylinositol 3-phosphate at the plasma membrane of unstimulated 3T3-L1 adipocytes (conditions under which phosphatidylinositol 3,4,5-triphosphate is not synthesized) |
3.1.3.36 | DNA and amino acid sequence detremination and analysis, expression of GFP-tagged synaptojanin-1 in CHO cells |
3.1.3.36 | expressed as a HIS-tagged fusion protein in 3T3-L1 adipocytes and in CHO-IR cells (Chinese hamster ovary cells stably transfected with the insulin receptor) |
3.1.3.36 | expressed in COS-7 cells and in Saccharomyces cerevisiae |
3.1.3.36 | expressed in Dictyostelium discoideum |
3.1.3.36 | expressed in Escherichia coli as a GST-fusion protein and as a Flag-tagged fusion protein in human hepatoma HuH-7 cells |
3.1.3.36 | expressed in Escherichia coli as a GST-tagged protein |
3.1.3.36 | expressed in Escherichia coli BL21(DE3) cells |
3.1.3.36 | expressed in HEK293 cells |
3.1.3.36 | expressed in murine 3T3-L1 preadipocytes |