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EC Number Cloned (Commentary) Reference
Show all pathways known for 3.1.3.27Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.27Escherichia coli strains lacking the original pgpA and/or pgpB genes 4778, 94810
Show all pathways known for 3.1.3.27Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.27expressed in Escherichia coli 730834
Show all pathways known for 3.1.3.27Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.27expressed in Escherichia coli BL21 (DE3) cells 682752
Show all pathways known for 3.1.3.27Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.27expressed in Escherichia coli BL21 (DE3) CodonPlus RIPL cells 730029
Show all pathways known for 3.1.3.27Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.27pgpB gene is cloned into the pET22b vector (Novagen) to generate the C-terminal His6-tagged protein. Mutants were generated using standard site-directed mutagenesis. The PgpB wild type and mutant proteins are expressed in Escherichia coli strain C41(DE3) 751064
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