EC Number |
---|
3.1.3.10 | - |
3.1.3.10 | expressed in Escherichia coli BL21(DE3), pET-22b(+) and pIP5 vectors |
3.1.3.10 | gene AgpEnB11, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) |
3.1.3.10 | gene agpP, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme lacking the signal peptide in Escherichia coli |
3.1.3.10 | in vivo cloning technique with plasmid Mu dII4042, expression in Escherichia coli strain SBS1295 |
3.1.3.10 | plasmid cloning with pEP1376, a derivate of pBR322 carrying a 1,8-kb DNA fragment containing the entire agp gene |