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Results 1 - 9 of 9
EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI 682686
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3expression in Escherichia coli 663535, 730342
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3expression of his-tagged YtqI under control of the arabinose-inducible promoter Para 682178
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3gene orn, detailed phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) 752033
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3gene orn, quantitative RT-PCR expression analysis 749595, 752038
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3gene orn, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha 752249
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3into pMCSG7 vector 682781
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3overexpression of the orn gene in Escherichia coli 134087, 134088
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3wild-type ornA and the ornA (D130A) point mutant are placed under the control of the strong, constitutive ermE promoter in the hygromycin-resistant, attP-integrative vector pIJ10275. The resultant plasmids (pJS93 and pJS94) introduced into Streptomyces coelicolor J3411 (DELTAornA::apr) by conjugation with Escherichia coli strain ET12567 (pUZ8002) to yield Streptomyces coelicolor J3411 (pJS93) and Streptomyces coelicolor J3411 (pJS94) 697978
Results 1 - 9 of 9