EC Number |
Reference |
---|
3.1.13.3 | expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI |
682686 |
3.1.13.3 | expression in Escherichia coli |
663535, 730342 |
3.1.13.3 | expression of his-tagged YtqI under control of the arabinose-inducible promoter Para |
682178 |
3.1.13.3 | gene orn, detailed phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
752033 |
3.1.13.3 | gene orn, quantitative RT-PCR expression analysis |
749595, 752038 |
3.1.13.3 | gene orn, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |
752249 |
3.1.13.3 | into pMCSG7 vector |
682781 |
3.1.13.3 | overexpression of the orn gene in Escherichia coli |
134087, 134088 |
3.1.13.3 | wild-type ornA and the ornA (D130A) point mutant are placed under the control of the strong, constitutive ermE promoter in the hygromycin-resistant, attP-integrative vector pIJ10275. The resultant plasmids (pJS93 and pJS94) introduced into Streptomyces coelicolor J3411 (DELTAornA::apr) by conjugation with Escherichia coli strain ET12567 (pUZ8002) to yield Streptomyces coelicolor J3411 (pJS93) and Streptomyces coelicolor J3411 (pJS94) |
697978 |