EC Number   |
|---|
   3.1.1.116 | gene DAGLA |
| 3.1.1.116 | gene DAGLA, DNA and amino acid sequence determination and analysis, sequence comparisons of the two isozymes, real-time RT-PCR analysis of DAGLalpha expression, recombinant expression of V5- and His-tagged isozyme DAGLalpha in COS-7 cells |
| 3.1.1.116 | gene DAGLA, located on the human chromosome 11q12.2 |
| 3.1.1.116 | gene Dagla, stable functional overexpression of V5-tagged DAGLalpha in COS-7 cell membranes, no DAGLalpha-induced change in COS-7 cell morphology or density is observed |
| 3.1.1.116 | gene DAGLalpha, identification of the core promoter region and regulatory elements of DAGLalpha, specificity protein 1 (Sp1), is the only factor that can bind to the GC-box, the core promoter contains both an enhancer and a suppressor region. The GC-box specifically promotes expression in NS cells. Functional transient recombinant expression of pGL3-DAGLalpha promoter constructs plus pRLTK Renilla plasmid in NS cells. Promoter activity study of promoters P1-P11, overview. The full-length DAGLa promoter (P1) is active in Cor-1, CGR8, and NS-5 NS cell lines. A comparative analysis of the eight most relevant promoter constructs in the Cor-1 cells shows that P6 is the smallest and most active promoter. Again, the activity of P6 is suppressed by the 3' region, as revealed with P8, P10, and in this case also P11. The loss of DAGLa promoter luciferase activity in Cor-1 cells is due to a loss of function specific to the GC-box. The core DAGLa promoter is under a different regulatory control in the different cell types and identify two independent elements that can specifically regulate expression in Cor-1 cells. The GC-box is required for DAGLa core promoter activity in Cor-1 cells but not in 3T3 cells |
| 3.1.1.116 | gene DAGLB, DNA and amino acid sequence determination and analysis, sequence comparisons of the two isozymes, recombinant expression of FLAG-tagged isozyme DAGLbeta in COS-7 cells |
| 3.1.1.116 | recombinant expression of DAGLalpha in HEK-293T cells |
| 3.1.1.116 | recombinant expression of the N-terminally GST-tagged catalytic domain (amino acids 228-672, GST-DAGLbeta CD) of DAGLbeta in Spodoptera frugiperda Sf9 insect cells using the baculovirus transfection system. Stable recombinant expression of V5-tagged DAGLbeta (V5beta4) in human U2OS osteosarcoma cells, which are genetically modified to express a CB1 receptor reporter construct (Tango CNR1-bla U2OS cells) |
