EC Number   |
|---|
   3.1.1.101 | - |
| 3.1.1.101 | cloned into a pET24b vector and transformed into Escherichia coli BL21-Gold(DE3) |
| 3.1.1.101 | expressed in Pichia pastoris. No significant differences between the expression of native Thc_Cut1 and of two glycosylation site knock out mutants (Thc_Cut1_koAsn and Thc_Cut1_koST) concerning the total extracellular protein concentration and volumetric activity are observed |
| 3.1.1.101 | expression in Escherichia coli |
| 3.1.1.101 | expression in Escherichia coli BL21 and in in Bacillus subtilis 168 |
| 3.1.1.101 | heterologous expression in Escherichia coli |
| 3.1.1.101 | the crystal structures of PETase in complex with substrate and product analogs provide critical information for understanding the mechanism of action of PETase. The wobbling conformation of W156 is closely related to the binding of substrate and product |
| 3.1.1.101 | the PETase from Ideonella sakaiensis without the N-terminal 29 amino acids is chemically synthesized, ligated into the pET32a vector, and expressed in Escherichia coli |
