EC Number   |
|---|
    2.8.3.12 | GCT expression in Escherichia coli |
| 2.8.3.12 | gctAB genes encoding the two subunits GctA and GctB are cloned, sequenced and overexpressed together in Escherichia coli DH5alpha, the two genes are located at the beginning of the hydroxyglutarate operon, GctA: 320 amino acids protein, GctB: 266 amino acids protein |
| 2.8.3.12 | overexpression of betaE54N and betaE54A mutant enzyme in Escherichia coli, fusion of the genes gctA and gctB, encoding the 2 subunits of Gct, yields GctF, which is expressed in Escherichia coli as a 65 kDa protein with 30% of wild-type activity |
| 2.8.3.12 | overexpression of wild-type and betaE54D mutant enzyme in Escherichia coli |
| 2.8.3.12 | wild-type and mutant genes gctA and gctB encoding the two subunits GctA and GctB are cloned and expressed together in Escherichia coli XL1-Blue |
