EC Number |
---|
2.7.8.33 | - |
2.7.8.33 | cloned into pET2130, generating the plasmid pWTM8. The wecA gene product is expressed with an N-terminal polyhistidine tag, expression in Escherichia coli C43(DE3) |
2.7.8.33 | design of a vector system to generate C-terminal protein fusions to the FLAG epitope, which is used to find conditions to monitor WecA by immunoblot analysis with anti-FLAG antibodies, to determine the correct site for initiation of translation, and to investigate the localization of hybrid proteins in the cytoplasmic membrane |
2.7.8.33 | detailed examination of the role of a conserved motif within the predicted large cytosolic loop |
2.7.8.33 | expressed in Escherichia coli MV501 cells |
2.7.8.33 | gene wecA, cloning in Escherichia coli strain DH5alpha |