EC Number   |
|---|
   2.7.8.11 | - |
| 2.7.8.11 | AtPIS1 cDNA cloned in Escherichia coli XL-1 Blue, functional complementation of Saccharomyces cerevisiae wild-type strain D501-1 |
| 2.7.8.11 | AtPIS1 cloned and expressed in Escherichia coli, a host naturally devoid of phosphatidylinositol |
| 2.7.8.11 | cDNA cloned by functional complementation in Saccharomyces cerevisiae |
| 2.7.8.11 | cDNA cloned by functional complementation of a Saccharomyces cerevisiae mutant |
| 2.7.8.11 | cloned and expressed in Escherichia coli |
| 2.7.8.11 | disruption of the PIS locus in the genome is lethal |
| 2.7.8.11 | expressed in Escherichia coli |
| 2.7.8.11 | from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP for introduction of C-terminal EYFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections |
| 2.7.8.11 | from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP or -CFP for introduction of C-terminal EYFP- or CFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections |
