EC Number |
---|
2.7.7.41 | - |
2.7.7.41 | expressed as GFP-fusion protein |
2.7.7.41 | expressed in CHO-1 cells |
2.7.7.41 | expressed in Escherichia coli BL21(DE3) cells |
2.7.7.41 | expression in COS cells |
2.7.7.41 | expression in COS-7 cell |
2.7.7.41 | expression in Escherichia coli. Fusion protein of glutathione S-transferase and residues 108 to 424 of the Solanum tuberosum enzyme is produced by Escherichia coli cells transformed with the isopropyl-beta-galactopyranoside inducible plasmid pGEXstCD1 |
2.7.7.41 | expression under the control of a GAL1 promoter in a null cds1 mutant yeast strain complements its growth defect and produces enzyme activity when induced with galactose |
2.7.7.41 | gene Tb927.7.220, DNA and amino acid sequence determination and analysis, phylogenetic tree. The protozoan homologous have N-terminal extensions as well as several insertions, making these gene products significantly larger than the average prokaryotic CDS. TbCDS gene Tb927.7.220 can complement the Saccharomyces cerevisiae cds1::KanMX knockout. Recombinant expression of HA-tagged enzyme in enzyme-deficient cells |
2.7.7.41 | recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells |