EC Number |
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2.7.4.16 | construction of the thiamine monophosphate kinase (thiL) overexpression plasmid, overexpression from pCLK710 |
2.7.4.16 | encoded by thiL, thiL is not transcriptionally regulated by thiamine or thiamine diphosphate |
2.7.4.16 | expression in Escherichia coli |
2.7.4.16 | expression in Escherichia coli BL21 |
2.7.4.16 | expression in Escherichia coli Rosetta BL21(DE3)R3 |
2.7.4.16 | gene THI80 or Pcal_0657, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |