EC Number |
---|
2.7.3.4 | - |
2.7.3.4 | expressed in Escherichia coli JM109 cells |
2.7.3.4 | expressed in Escherichia coli TB1 cells |
2.7.3.4 | expression in Escherichia coli TB1 |
2.7.3.4 | gene CLF_107872, DNA and amino acid sequence determination and analysis, sequence comarisons and phylogenetic analysis, the coding region of the cDNA of D1D2 is cloned into the pMAL-c2 vector and expressed as maltose binding protein fusion protein in Escherichia coli strain TB1, quantitative real-time PCR enzyme expression analysis. Recombinant expression of N-terminally GST-tagged enzyme D1 and D2 mutants in Escherichia coli strain BL21(DE3) pLysS |
2.7.3.4 | gene PHYSODRAFT_566100, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminal His6-PsPK protein in Escherichia coli strain Rosetta 2 (DE3) |
2.7.3.4 | recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |
2.7.3.4 | sequence comparisons, recombinant expression of enzyme domain1 and domain2 mutants in Escherichia coli strain TB1 |
2.7.3.4 | the maltose binding protein-fused enzyme is expressed in Escherichia coli BL21(DE3) cells |