EC Number |
---|
2.7.11.7 | - |
2.7.11.7 | cells overexpressing GFP-GEF have both unphosphorylated and autophosphorylated MHCK A |
2.7.11.7 | cloning of wild-type and mutants of the catalytic domain of myosin I heavy chain kinase as His-tagged proteins and expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system |
2.7.11.7 | construction of His-tagged or FLAG-tagged N-terminal enzyme peptides by site directed mutagenesis for introduction of start and stop codons, expression in Escherichia coli BL21(DE3) |
2.7.11.7 | construction of His-tagged or GFP fusion proteins with the full length enzyme or enzyme fragments, expression in Dictyostelium discoideum cells |
2.7.11.7 | DNA and amino acid sequence determination and analysis |
2.7.11.7 | DNA sequence determination and analysis |
2.7.11.7 | DNA sequence determination and analysis, gene MHCK possesses all of the domains characteristic of members of the protein kinase C family |
2.7.11.7 | expressed in Escherichia coli BL21(DE3) cells |
2.7.11.7 | expression of FLAG-tagged T825A and T825E enzyme mutants in Dictyostelium discoideum cells, expression of His-tagged wild-type and mutant MHCK A alpha-kinase domains in Escherichia coli strain BL21(DE3) |