EC Number   |
Reference |
|---|
   2.7.11.21 | - |
491390, 491394, 491395, 491416, 679094, 705070 |
| 2.7.11.21 | a DNA fragment encoding the N-terminus of the TbPLK gene product is cloned into the tetracycline-inducible RNAi vector pZJM for knockdown of TbPLK expression, for tagging proteins TbPLK is cloned into the pC-EYFP-Bla vector, a plasmid for expressing glutathione S-transferase-TbPLK is constructed by cloning a DNA fragment containing full-length TbPLK into the vector pET41a |
703519 |
| 2.7.11.21 | a full-length Myc-PLK1 plasmid is used, full-length PLK1, cloned into pDON207, is inserted into a pCINeo vector, pFLAG-PLK1-PBD and pEGFP-PLK1-PBD constructs are generated |
704521 |
| 2.7.11.21 | a GST-fusion protein is generated |
694305 |
| 2.7.11.21 | determination of nucleotide sequence of cDNA |
491397 |
| 2.7.11.21 | developmental expression analysis of the highly conserved enzyme |
662998 |
| 2.7.11.21 | DNA sequence determination, transient expression of FLAG-tagged wild-type and mutant enzymes in 293T cells, co-expression witg GFP, overexpression induces wild-type protein kinase Chk2 phosphorylation, but does not increase phosphorylation of Chk2 mutant T68A |
662181 |
| 2.7.11.21 | EGFP-tagged Cdc5 protein is expressed by high copy plasmid pRS424, 425 or 426 under control of authentic promoter |
694807 |
| 2.7.11.21 | expressed in baculovirus-infected sf9 insect cells |
761318 |
| 2.7.11.21 | expressed in Escherichia coli BL21(DE3) pLysS as a GST fusion protein, 293T cells co-transfected with FLAG-PLK1 and GFPHsCdc14A, GST-tagged PLK1 kinase expressed in insect, HeLa cells transiently transfected to express GFP-HsCdc14A and FLAG-PLK1 |
680810 |
