EC Number   |
|---|
   2.7.1.149 | - |
| 2.7.1.149 | expressed in a enzyme-deficient mutant of Drosophila melanogaster |
| 2.7.1.149 | expressed in Escherichia coli BL21 cells |
| 2.7.1.149 | expressed in Escherichia coli BL21(DE3) cells |
| 2.7.1.149 | expressed in Escherichia coli BL21(DE3/pLysS) cells |
| 2.7.1.149 | expression of EGFP-tagged isozymes PIPKIIbeta and PIPKIIalpha in SW480-ADH cells cytosol and nucleus or cytosol, respectively |
| 2.7.1.149 | isozyme PIP4K IIbeta, functional expression in enzyme-deficient CHO-IR cells |
| 2.7.1.149 | overexpression in COS-7 cells |
| 2.7.1.149 | PI5P4Kalpha is predominantly in the cytoplasm, principally cytosolic, when recombinantly expressed |
| 2.7.1.149 | PIP5K2 genes are amplified from a whole human brain marathon-ready cDNA library using gene-specific primers. Recombinant protein is expressed from Escherichia coli harbouring PIP5K2s in pET-32a. PCR products are cloned, via incorporated HindIII and BamHI restriction sites, into appropriate plasmid vectors. HeLa, HEK 293, COS-7 and NRK cells are transiently transfected with plasmid constructs. |
