EC Number |
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2.6.1.B21 | construction of the pET28a vector carrying a codon-optimized gene of an engineered D-selective omega-transaminase (omega-TA) ARTAmut from Arthrobacter sp. (ARTAmut), recombinant expression of His-tagged omega-TA in Escherichia coli strain BL21(DE3) |
2.6.1.B21 | gene ilvE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
2.6.1.B21 | gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV proteae cleavage site in Escherichia coli strain BL21(DE3)pLys |
2.6.1.B21 | gene ilvE1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
2.6.1.B21 | gene pabC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
2.6.1.B21 | recombinant expression in Escherichia coli strain BL21(DE3) |
2.6.1.B21 | recombinant expression of C-terminally His-tagged (R)-amine transaminase, a engineered modified DATA mutant enzyme, in Escherichia coli strain BL21(DE3) |
2.6.1.B21 | recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLysS |
2.6.1.B21 | sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) |