EC Number   |
Reference |
|---|
    2.6.1.21 | - |
636744, 636745, 636751 |
| 2.6.1.21 | expressed in Escherichia coli |
721250 |
| 2.6.1.21 | expression in Escherichia coli |
739709 |
| 2.6.1.21 | expression of wild-type, L201A and L201W mutant enzymes in Escherichia coli |
636743 |
| 2.6.1.21 | gene DAAT, construction of expression plasmid pENTR-AtDAT1(Col-0), pENTR-AtDAT1(A77T), pENTR-AtDAT1(T303S), and pENTR-AtDAT1(Ler), recombinant expression of C-terminally GST and N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) RIL. The wild-type and mutant enzymes are transfected into Arabdiospsis thaliana and Nicotianan benthamiana plants via the Agrobacterium tumefaciens strain pMP90-RK GV3101 transfection system, leaf infiltration resulting in insertion lines, SALK_011686 and SALK_111981, denoted as dat1-1 and dat1-2, respectively. Histochemical staining of GUS activity in seedlings and adult plants incubated overnight at 37°C in this buffer containing 1 mM X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid) and 0.5 mM K3Fe(CN)6. RT-PCR enzyme expression analysis |
759259 |
| 2.6.1.21 | gene Dret_1107, construction of the synthetic gene coding the transaminase Dret from Desulfohalobium retbaense, recombinant expression |
759812 |
| 2.6.1.21 | gene FQP89_01185, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3) |
759235 |
| 2.6.1.21 | recombinant expression of C-terminally His-tagged wild-type DATA enzyme and mutant enzymes in Escherichia coli strain BL21(DE3) |
758564 |
