EC Number   |
Reference |
|---|
    2.6.1.13 | - |
637134, 637135, 637138, 637139 |
| 2.6.1.13 | a recombinant seedling OAT is obtained by cDNA expression in Escherichia coli and its substrate specificity is measured, the enzyme is found to be strictly specific for L-ornithine showing practically no activity with putrescine, 1,3-diamimopropane and 4-aminobutyrate |
706356 |
| 2.6.1.13 | calmodulin-binding peptide fusion protein |
637128 |
| 2.6.1.13 | expressed in Escherichia coli |
674489 |
| 2.6.1.13 | expressed in Escherichia coli as a His-tagged fusion protein |
721755 |
| 2.6.1.13 | expression in Escherichia coli |
688463, 689633 |
| 2.6.1.13 | expression of the entire human gene in Escherichia coli |
706356 |
| 2.6.1.13 | fusion with maltose binding protein |
637140 |
| 2.6.1.13 | gene TaOAT-5AL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure, and phylogenetic tree. Two transcript variants of TaOAT-5AL are revealed, named TaOAT-5AL-1 and TaOAT-5AL-2 and characterized by 1497 bp and 1287 bp in cDNA length, respectively. Compared to TaOAT-5AL-2, TaOAT-5AL-1 contains an additional 120-bp insertion encompassing an in-frame stop codon, which resulted in a premature protein. The additional insertion is genotypically confirmed by sequencing results from six cultivars. The TaOAT-5AL-1 gene transcript variant contains in-frame stop codon that causes the incomplete translation of protein. Recombinant overexpression of TaOAT-AL-2 in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in Triticum aestivum plants in mitochondria, quantitative reverse transcription PCR (qRT-PCR) analysis |
758976 |
| 2.6.1.13 | gene TaOAT-5BL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure and phylogenetic tree, recombinant overexpression in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in triticum aestivum plants in mitochondria quantitative reverse transcription PCR (qRT-PCR) analysis |
758976 |
