EC Number   |
|---|
   2.5.1.67 | a c98f chimera is constructed by replacing the first 98 residues in Artemisia tridentata subsp. spiciformis farnesyl diphosphate synthase with the corresponding sequence from chrysanthemyl diphosphate synthase, the enzymes are cloned in Escherichia coli hosts |
| 2.5.1.67 | expression of mutant chimeric CPPase-FPPase enzyme in Escherichia coli |
| 2.5.1.67 | gene CDS, dNA and amino acid sequence determination and analysis, sequence comparisons, recombinant CDS overexpression under the control of chrysanthemum RbcS1 promoter in transgenic Nicotiana tabacum cv. Samsun plants, in leaf chloroplasts, using Agrobacterium tumefaciens strain AGL-0 transfection method, the expression leads to emittation of chrysanthemol. Recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(AI) |
| 2.5.1.67 | gene CDS, real-time PCR enzyme expression analysis |
| 2.5.1.67 | gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization |
| 2.5.1.67 | phage lambda cDNA library screening, DNA and amino acid sequence determination and analysis, functional overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90 |
