EC Number |
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2.5.1.63 | expression in Escherichia coli |
2.5.1.63 | expression in Escherichia coli BL21 |
2.5.1.63 | expression in Escherichia coli BL21(DE3) |
2.5.1.63 | expression in Escherichichia coli |
2.5.1.63 | expression of His-tagged enzyme in Escherichia coli BL21(DE3) pLysS with plasmid pET28b+ |
2.5.1.63 | gene flA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type His6-tagged enzymes in Escherichia coli strain BL21(DE3) |
2.5.1.63 | gene flA, DNA sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) and in strain B834(DE3), the latter results in the selenomethionine labeled enzyme |
2.5.1.63 | gene flA, genetic structure and organization |
2.5.1.63 | gene flA, genetic structure and organization, the flA gene is located in close proximity to the corresponding flB gene |
2.5.1.63 | gene flA, located at the center of the 10 kb gene Spencer cluster, genetic structure and organization, the f lA gene is located in close proximity to the corresponding f lB gene. Funtional recombinant expression in Salinispora tropica using gene replacement and replacing the gene salL that encodes chlorinase, EC 2.5.1.94, by gene flA from Streptomaces cattleya. The mutated Salinispora tropica strain produces fluorosalinosporamide |