EC Number |
---|
2.5.1.59 | - |
2.5.1.59 | alpha-subunit encoded by RAM2 and beta-subunit encoded by CDC43 translationally coupled by overlapping the RAM-CDC43 stop-start codons and by locating a ribosome-binding site near the 3' end of RAM2, recombinant enzyme overproduced in Escherichia coli |
2.5.1.59 | alpha-subunit encoded by RAM2 cloned to the pFC vector, which has an chloroamphenicol resistance gene and beta-subunit encoded by CAL1 cloned to the pFlag vector, which has an ampicillin resistance gene, these two recombinant enzymes are cotransformed and expressed in Escherichia coli |
2.5.1.59 | coexpression of two subunits of enzyme by Sf9 cells infected with recombinant baculovirus |
2.5.1.59 | expressed in Escherichia coli |
2.5.1.59 | expressed in Escherichia coli BL21 (DE3) and Sf9 insect cells |
2.5.1.59 | expressed in Escherichia coli strain BL21(DE3) GGPT/pET23a |
2.5.1.59 | expressed in Sf9 insect cells |
2.5.1.59 | gene cdc43, encoding the Ggtase-I beta-subunit, DNA and amino acid sequence determination and analysis, recombinant expression of wild-ype and mutant enzymes |
2.5.1.59 | identification of the cwp1+ gene, which encodes the alpha-subunit of enzyme, coexpression of cwp1p and cwg2p, beta-subunit, in Escherichia coli |