EC Number   |
|---|
    2.4.2.9 | cloning from a fetal brain cDNA library, DNA and amino acid sequence determination, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain M15, phylogenetic analysis, expressionin AD293 cells, tissue and subcellular expression pattern |
| 2.4.2.9 | co-expression of gene CodA, encoding cytosine deaminase, and gene upp, encoding uracil phosphoribosyl transferase, renders HeLa cells sensitive to 5-fluorocytosine, converted to toxic 5-fluorouracil by cytosine deaminase, and enhanced a bystander effect mediated by a gap junction mechanism, 1% cytosine deaminase+-UPP+ cells are able to kill 40% of the cell population if the cells are communicating |
| 2.4.2.9 | coexpression of the uracil phosphoribosyltransferase gene with a chimeric human nerve growth factor receptor/yeast cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells, CEM cells, exposed to 5-fluorocytosine |
| 2.4.2.9 | cytosine deaminase-UPRT-red fluorescence protein fusion proteins are expressed in Rattus norvegicus R-3327-AT cells |
| 2.4.2.9 | DNA and amino acid sequence determination and analysis of the natural chimeric gene UK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT, expressionin Escherichia coli, transient expression as C-terminally GFP-tagged enzyme in Arabidopsis thaliana mesophyll protoplasts, 5-FU sensitivity of the Escherichia coli upp mutants is influenced by the expression of AtUK/UPRT1, as well as the 5-FU and 5-FD sensitivity of Escherichia coli upp-udk mutants, overview |
| 2.4.2.9 | DNA and amino acid sequence determination and analysis, overexpression in enzyme-deficient Escherichia coli |
| 2.4.2.9 | DNA sequence determination and analysis, gene upp belongs to purMN operon, complementation of deficient Escherichia coli strain |
| 2.4.2.9 | DNA sequence determination, functional overexpression in Escherichia coli BL21(DE3)plyS |
| 2.4.2.9 | expressed in A2780 cells, A2780/Taxol cells, Skov3 cells, and Skov3/Taxol cells |
| 2.4.2.9 | expressed in AsPC-1, BxPC3, CaPan-1, MIA, PaCa-2, Panc-1, and HT-29 cell lines |
