EC Number   |
Reference |
|---|
   2.4.1.B72 | gene dpy-19, recombinant expression in Drosophila melanogaster S2 cells, coexpression with receptor proteins UNC-5 or MIG-21 |
736804 |
| 2.4.1.B72 | gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells |
759311 |
| 2.4.1.B72 | gene dpy19, recombinant enzyme expression in Drosophila melanogaster S2 cells |
760067 |
| 2.4.1.B72 | gene Dpy19l1, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4. Overexpression of DPY19L1 results in detection of TSR1 peptides with a maximum of two C-mannoses |
760067 |
| 2.4.1.B72 | gene Dpy19l3, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4. The ratio of this C-mannosylated peptide to the nonmannosylated peptide is higher in cells overexpressing DPY19L3 than in wild-type cells |
760067 |
| 2.4.1.B72 | gene Dpy19l4, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4 |
760067 |
| 2.4.1.B72 | recombinant expression in S2 cells, coexpression with substrate Rspo1 |
736780 |
| 2.4.1.B72 | transient recombinant expression of wild-type and mutant enzymes in Drosophila melanogaster S2 cells or HEK-293T cells fused to Gaussia luciferase or tagged with myc or myc-His6 tags, quantitative RT-PCR expression analysis |
759174 |
