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Results 1 - 7 of 7
EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.85cloning of a full-length cDNA encoding enzyme, expression in Escherichia coli JM109, 492-amino acids translation product
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.85expressed in Arabidopsis thaliana
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.85expressed in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.85gene UGT85B1, DNA and amino acid sequence determination and analysis of wild-type and mutant tcd2 genes. The CYP79A1, CYP71E1 and UGT85B1 genes required for dhurrin synthesis are clustered
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.85mutant enzymes, expression in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.85recombinant expression in Nicotiana tabacum chloroplasts, coexpression with CYP79A1 and CYP71E1 linked in a tricistronic operon construct with the essential regulatory elements
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.85transgenic Arabidopsis thaliana plants expressing the entire biosynthetic pathway for the tyrosine-derived cyanogenic glucoside dhurrin as accomplished by insertion of CYP79A1, CYP71E2, and UFT85B1 are shown to accumulate 4% dry-weight dhurrin with marginal inadvertent effects on plant morphology, free amino acid pools, transcriptome, and metabolome
Results 1 - 7 of 7