EC Number |
---|
2.4.1.232 | ANL1 and OCH1 gene |
2.4.1.232 | as a fusion with the with green fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned |
2.4.1.232 | as a fusion with the with red fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned |
2.4.1.232 | into the pGEMT-Easy vector |
2.4.1.232 | MNN9 |
2.4.1.232 | MNN9 gene encoding Mnn9p is cloned and sequenced |
2.4.1.232 | OCH1 gene encodes Och1p, an alpha-1,6-mannosyltransferase |
2.4.1.232 | och1+ gene encodes alpha-1,6-mannosyltransferase, recombinant expression and cloning, och1+ gene knockout fission yeast |
2.4.1.232 | och1+ gene, sequencing |
2.4.1.232 | recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli BL21(DE3) pLysS, expression of mutant enzymes in Saccharomyces cerevisiae strain BY4741 DELTAMNN9 |