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Results 1 - 8 of 8
EC Number Cloned (Commentary) Reference
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227expressed in Escherichia coli cells BL21-DE3-pLysS 488971, 488973, 488974, 488976, 488977
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227gene murG, expression as His6-tagged enzyme in Escherichia coli strain C43 721824
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227gene murG, expression of C-terminal YFP-tagged enzyme in a murG-deficient strain of Bacillus subtilis 735983
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227into the vector pQE60 for expression of MurG in Escherichia coli JM109 cells 691120
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227MurG can be displayed in its active form on phage. Cloned from a pET-21a expression vector (Novagen) into the pFAB5cHis.TT.HUI phagemid vector as a fusion to the 3’-end of a pelB leader sequence and the 5’-end of a truncated gIII 673085
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227the coding sequence is amplified from the Escherichia coli K12 chromosome and cloned for expression of His-tagged MurG in JM109 cells 676084
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid is transformed and expressed in Escherichia coli NiCo21(DE3) competent cells 758570
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid was transformed and expressed in Escherichia coli NiCo21(DE3) competent cells 758570
Results 1 - 8 of 8