EC Number   |
|---|
   2.4.1.227 | expressed in Escherichia coli cells BL21-DE3-pLysS |
| 2.4.1.227 | gene murG, expression as His6-tagged enzyme in Escherichia coli strain C43 |
| 2.4.1.227 | gene murG, expression of C-terminal YFP-tagged enzyme in a murG-deficient strain of Bacillus subtilis |
| 2.4.1.227 | into the vector pQE60 for expression of MurG in Escherichia coli JM109 cells |
| 2.4.1.227 | MurG can be displayed in its active form on phage. Cloned from a pET-21a expression vector (Novagen) into the pFAB5cHis.TT.HUI phagemid vector as a fusion to the 3-end of a pelB leader sequence and the 5-end of a truncated gIII |
| 2.4.1.227 | the coding sequence is amplified from the Escherichia coli K12 chromosome and cloned for expression of His-tagged MurG in JM109 cells |
| 2.4.1.227 | the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid is transformed and expressed in Escherichia coli NiCo21(DE3) competent cells |
| 2.4.1.227 | the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid was transformed and expressed in Escherichia coli NiCo21(DE3) competent cells |
