EC Number   |
|---|
   2.4.1.140 | expression in Escherichia coli |
| 2.4.1.140 | expression in Solanum tuberosum. The Asr transcript levels correlated well with alternan accumulation in tuber juices |
| 2.4.1.140 | gene asr, recombinant expression of wild-type enzyme and truncated mutants in Escherichia coli strain BL21(DE3) |
| 2.4.1.140 | gene Lcalt, rcombinant expression in Escherichia coli strain BL21(DE3) |
| 2.4.1.140 | recombinant expression of the large alternansucrase (2057 amino acids) is hindered in Escherichia coli due to poor enzyme solubility and protein degradation. A truncated variant deleted of the APY repeats but harboring four C-terminal CW-like repeats display a high specific activity and the same specificity of product synthesis as the native enzyme. It is more soluble and suffers less degradation than full length alternansucrase |
