EC Number   |
|---|
    2.4.1.129 | - |
| 2.4.1.129 | a soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain |
| 2.4.1.129 | activity of PBP1a or PBP1b can be measured in membranes by cloning the PBP into an Escherichia coli ponB::Spcr strain |
| 2.4.1.129 | construction of expression plasmids allowing the production of native PBP1B or PBP1B variants with an inactive transpeptidase or transglycosylase domain or both. Overproduction of the inactive PBP1B variants, but not of the active proteins, causes lysis of wild-type cells. Cells became tolerant to lysis by inactive PBP1B at a pH of 5.0 |
| 2.4.1.129 | enzyme overexpression in Escherichia coli imp mutant strain |
| 2.4.1.129 | Escherichia coli structural gene mrcB, recloned from plasmid pLC19-19 to high copy number plasmid pBr322, yielding plasmid pTM13 |
| 2.4.1.129 | expressed in Escherichia coli BL21(DE3) cells |
| 2.4.1.129 | expressed in Escherichia coli BL21(lambdaDE3)-RIL cells |
| 2.4.1.129 | expressed in Escherichia coli Rosetta lambdaDE3 cells |
| 2.4.1.129 | expressed in Escherichia coli Top10 cells |
