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Results 1 - 4 of 4
EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.B25DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis 722034
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.B25gene pksE, DNA and amino acid sequence determination and analysis 757727
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.B25recombinant expression of the gene encoding wild-type enzyme OKS in Aspergillus nidulans strain NID2252 controlled by the Aspergillus nidulans gpdA promoter as a single copy. Construction of a strain co-expressing OKS with ZhuI, which encodes the cyclases that catalyzes formation of the C7-C12 first ring closure. This strain shows 2.5, 2.2, and 2.1fold increases in flavokermesic acid, SEK4, and dehydro-SEK4 levels, respectively, compared to expression of OKS alone. This increase is 60% higher than what is observed with the strains expressing OKS in combination with either ZhuI (UniProt ID Q9F6D3) or ZhuJ (UniProt ID Q9F6D2), respectively, indicating that the cyclase and aromatase, in an additive manner, are able to increase the flux in the artificial de novo pathway towards the desired product, flavokermesic acid 758420
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.B25transient functional recombinant expression in Nicotiana benthamiana, coexpression with genes StZhuI and StZhuJ from Streptomyces sp., and DcUGT2 from Dactylopius coccus 756452
Results 1 - 4 of 4