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Results 1 - 10 of 11 > >>
EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42enzyme cloned from a cDNA library matching EST-clones with peptides from rabbit peroxisomal enzyme, DNA sequence determination 486973
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42expressed in HEK-293E cells and Pichia pastoris KM71H MutS strain 755947
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42expressed in Saccharomyces cerevisiae cells 758156
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42expression in CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or dcreased in both cases 659671
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42expression of the human enzyme encoding cDNA in a CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or decreased in both cases 659671
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42functional expression in Saccharomyces cerevisiae 486975
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42functional expression in Saccharomyces cerevisiae, DNA and amino acid sequence determination 486975
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42gene DAT, DNA amd amino acid sequence determination and analysis, DAT functionally complements the lethality resulting from the loss of both dihydroxyacetone phosphate and glycerol-3-phosphate acyltransferase activities in yeast, recombinant DAT exhibits biochemical properties similar to those of the native enzyme of the promastigote stage parasites 674766
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42gene TTHERM_00221020, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant transient expression of GFP-tagged truncated bifunctional enzyme in Nicotiana tabacum cv. Petit Havana epidermis, using the Agrobacterium tumefaciens GV3101 strain and used for transient expression system, and transgenic expression in Saccharomyces cerevisiae, the recombinant enzyme's N-terminal FAR-like domain produces both 16:0 and 18:0 fatty alcohols, whereas the C-terminal acyltransferase-like domain is able to rescue the lethal phenotype of the Saccharomyces cerevisiae double mutant cmy228 (gat1DELTAgat2DELTA). Coexpression in Saccharomyces cerevisiae with the alkyl-dihydroxyacetone phosphate synthase from Tetrahymena thermophila results the detection of various glycerolipids with an ether bond. In yeast, GAT1 and GAT2 are the only two acyltransferases acylating the sn-1 position of G3P and DHAP, thus being essential to initiate glycerolipid biosynthesis 736453
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.42genes GAT1 and GAT2, expression in Escherichia coli DH5alpha, Gat1 and Gat2 proteins are glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferases, amino acid sequence analysis 486391
Results 1 - 10 of 11 > >>